This article presents a reproducible bioinformatics workflow integrating public transcriptomic datasets, machine learning, external validation, quantitative reverse transcription PCR, Connectivity Map screening, and molecular docking to identify pulmonary hypertension biomarkers and candidate therapeutic compounds.

bioinformatics-machine-learning-identification-pulmonary-hypertension

Bioinformatics and Machine-Learning Identification of Pulmonary Hypertension Biomarkers and Candidate Therapeutic Compounds

This study aimed to identify pulmonary hypertension (PH)-associated molecular biomarkers and candidate small-molecule compounds using public ...

Here, we constructed a multi-dimensional network of &#34;component-target-pathway-disease&#34;, integrating network pharmacology screening, molecular docking, and LPS-induced mouse model validation. By establishing clear key parameters, it enables the efficient identification of the active components of Tong ethnic medicine, Lengyuxiao Tang, and the verification of a repeatable process.

computer-aided-active-ingredients-screening-validation-dong-medicine

Computer-Aided Active Ingredients Screening and Validation of Dong Medicine for Fever and Cough Exploration

To systematically screen and verify the active components in the Dong ethnic medicine Lengyuxiao Tang (LYXT; Semen Pharbitidis, Verbena officinalis L., ...

This protocol investigates the heterochromatin distribution in eight populations of Lycoris aurea (L&#8242;Her) Herb, using combined PI and DAPI chromosome staining, fluorescence in situ hybridization (FISH) with 18S-5.8-26S rRNA gene regions (45S rDNA regions), and 5S ribosomal DNA probes.

comparative-karyotype-analysis-lycoris-aurea-herb-populations-using

Comparative Karyotype Analysis of Lycoris aurea Herb Populations Using Fluorochrome Banding and 45S and 5S rDNA-FISH

To understand karyotype variation in eight populations, detailed karyotypes were meticulously established using chromosomal measurements, fluorescence ...

Institution - Huaihua University

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