<p class="jove_content">Here, we propose a simple protocol combining metabolic oligosaccharide engineering, click chemistry, and expansion microscopy that allows bioimaging of intracellular sialylated <em>N</em>-glycoproteins with improved resolution using routine microscopy equipment.</p>

visualizing-intracellular-sialylation-with-click-chemistry-expansion

Visualizing Intracellular Sialylation with Click Chemistry and Expansion Microscopy

Metabolic labeling techniques allow the incorporation of bioorthogonal reporters into glycans, enabling the targeted bioconjugation of molecular dyes ...

Institution - Univ. Lille, CNRS, UMR 8520 - IEMN – Institut d’Electronique, de Microelectronique et de Nanotechnologie

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