This single-center retrospective study analyzes de-duplicated Pseudomonas aeruginosa (PA) isolates from 2023 to 2025 and examines resistance trends before and after a four-component antimicrobial stewardship intervention in the Resp-TB.

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Trends in Pseudomonas aeruginosa Resistance After a Four-Dimensional Antimicrobial Stewardship Intervention

This single-center retrospective study analyzed de-duplicated Pseudomonas aeruginosa isolates collected at Huaian Municipal Infectious Diseases Hospital ...

Polygonal gating (PGG) and cruciform gating (CFG) are flow cytometry methods for detecting sperm DNA integrity. PGG measures the DNA fragmentation index (DFI) and the high DNA staining index (HDS). CFG reports severe DFI, mild DFI, and overall DFI. This study aims to compare the results and correlations between them.

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Comparison of Sperm DNA Analysis Outcomes Using Different Gating Strategies in Flow Cytometry

Sperm DNA integrity is a key factor in ensuring successful fertilization, embryo development, and ongoing&#160;pregnancy. Sperm chromatin structure ...

This protocol describes a method for screening high-quality sperm with a low DNA fragmentation index by utilizing sperm motility characteristics and thigmotaxis. The method employs a U-shaped horizontal swimming lane, enabling high-quality sperm to reach the opposite side through buffer droplets, while dead sperm, cell debris, and impurities are excluded.

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U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

Human semen is a complex mixture comprising progressively motile spermatozoa, non-progressively motile spermatozoa, immotile spermatozoa, cell debris, and ...

Institution - The Affiliated Huai'an No. 1 People's Hospital of Nanjing Medical University

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