Overview

This protocol outlines the culture of freshly obtained (passage 0) keratinocytes for use in live cell imaging.

Key Study Components

What are keratinocytes?

Keratinocytes are the predominant cell type in the outer layer of the skin, responsible for forming a protective barrier.

Why is live cell imaging important?

Live cell imaging allows researchers to observe cellular processes in real-time, providing insights into cell behavior and function.

What is passage 0 in cell culture?

Passage 0 refers to cells that are freshly isolated and have not yet been subcultured, maintaining their original characteristics.

How are keratinocytes cultured?

Keratinocytes are cultured in specific media that support their growth and maintain their viability.

What applications do cultured keratinocytes have?

Cultured keratinocytes are used in research related to skin biology, wound healing, and drug testing.