Overview

This research introduces a novel method for simultaneous identification and genotyping of mouse pups aged 7 to 14 days using a pinna-edge biopsy (PEB). The technique allows researchers to acquire genetic information earlier than traditional methods, which typically start at 14 days or later.

Key Study Components

Research Area

Genetics
Developmental biology

Background

Traditional identification methods start at 14 days or later.
Current PEB method provides nine ID patterns, sufficient for litters up to nine pups.

Methods Used

The protocol utilizes a pinna-edge biopsy on mouse pups to harvest tissue for genotyping.
The biological system involves post-natal day (PND) 7 mice.
Important technologies include clean dissection scissors and fine forceps.

Main Results

Pinna biopsy patterns were identifiable up to postnatal day 63.
Both male and female mice showed significant body weight increases from postnatal day 7 to 63.
The study confirms that the right horizontal biopsy angle appears more horizontal over time as the ear matures.

Conclusions

This study demonstrates an effective approach for early genetic identification and genotyping in mouse pups.
The method holds significance for advancing genetics research in developmental biology.

Frequently Asked Questions

What age of mouse pups can be used for PEB?

The protocol is designed for mouse pups aged 7 to 14 days.

How many ID patterns does the PEB method provide?

The method provides nine ID patterns for identification.

What is the primary biological system used in this research?

The primary biological system involves post-natal day 7 mice.

What instruments are used for conducting the PEB?

Clean dissection scissors and fine forceps are utilized for the biopsy.

How long are the biopsy patterns identifiable?

The pinna biopsy patterns remain identifiable up to postnatal day 63.

Did the study observe any changes in body weight of the mice?

Yes, both male and female mice showed significant increases in body weight from postnatal day 7 to 63.

Institution - Massachusetts General Hospital Center for Comparative Medicine

Articles Published in JoVE