This procedure aims to identify metabolites transferred from sperm to oocytes during fertilization by using stable isotope labeling with 2H2O or U13C Glucose followed by metabolomic analysis. Deuterium-labeled sires are mated with unlabeled dams. Collected pre-implantation embryos are analyzed by mass spectrometry to sort paternal (deuterated) from maternal (non-deuterated) metabolites.

stable-isotope-vivo-labeling-for-mass-spectrometry-identification

Stable Isotope In-Vivo Labeling for Mass-Spectrometry Identification of Paternal Metabolites Transferred from Sperm to Oocyte During Fertilization

Paternal contribution to embryo genetics has so far been limited to allelic sequences for decades. A decade of results suggests, instead, that epigenetic ...

Institution - Institute of Molecular Biology and Genetics Research

Articles Published in JoVE