Here, we present a protocol to visualize and standardize resazurin microtiter assay (REMA) and its checkerboard variation for evaluating the drug susceptibility and interaction profiles of Mycobacterium tuberculosis. This method provides insights, in vitro, for optimizing drug combinations to be used in treatment regimens and combating multidrug-resistant tuberculosis.

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Detection of Drug Susceptibility and Drug Interactions in Mycobacterium tuberculosis using Resazurin Microtiter and Checkerboard Assay

The emergence of multidrug-resistant tuberculosis (MDR-TB) necessitates rapid and accurate drug susceptibility testing (DST) methods to guide effective ...

This protocol separates insoluble cell wall and membrane proteins into simple fractions (1-5 proteins) using preparative isoelectric focusing (IEF) based on isoelectric point, followed by separation by molecular weight. The resulting fractions can be used directly for immunological and proteomic analysis without further purification.

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Separation and Fractionation of Cell Wall and Cell Membrane Proteins from Mycobacterium tuberculosis for Downstream Protein Analysis

Mycobacterium cell wall and membrane proteins, which play a central role in tuberculosis pathogenesis, were successfully separated using preparative ...

This protocol describes the culturing, harvesting, and concentration of secreted proteins of Mycobacterium tuberculosis. Culture filtrate proteins (CFPs) are quantified, separated into 20 fractions by isoelectric focusing, and further resolved into 30 fractions using a preparative SDS-PAGE and an electroelution system.

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Institution - National Institute for Research in Tuberculosis

Articles Published in JoVE