Multi-chamber social preference tasks have been adapted from rodents to zebrafish, facilitating the study of social behavior in model organisms. This protocol validates a low-cost 3-chamber open-tank task using webcams and open-source software to measure zebrafish social preference, making it accessible to undergraduate research and teaching.

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Low-Cost Alternative for Examining Social Preference in Zebrafish (Danio rerio) Using the 3-Chamber Open-Tank Free-Swim Task

Understanding social behavior is a core pursuit in neurobehavioral research, often studied using social preference tasks. These tasks, originally ...

Here, we present a protocol to use thiazole orange for the detection of DNA in gel electrophoresis experiments. The use of thiazole orange allows elimination of ethidium bromide, and fluorescence detection can be achieved with either UV or blue light.

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DNA Electrophoresis Using Thiazole Orange Instead of Ethidium Bromide or Alternative Dyes

DNA gel electrophoresis using agarose is a common tool in molecular biology laboratories, allowing separation of DNA fragments by size. After separation, ...

The Open-field Tower Maze (OFTM) is a maze created to study the behavioral and neural mechanisms of spatial learning (e.g., place- or response-learning) in rats. This maze is especially useful for experimenters who want to use a non-stressful maze paradigm to investigate spatial learning in their research.

place-and-response-learning-in-the-open-field-tower-maze

Place and Response Learning in the Open-field Tower Maze

This protocol describes how the Open-field Tower Maze (OFTM) paradigm is used to study spatial learning in rodents. This maze is especially useful for ...

Institution - Christopher Newport University

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