This protocol describes the design and use of an isolation chip (iChip) for in situ cultivation of recalcitrant soil microbes in their natural environment. It also describes the in situ cultivation of antimicrobial-producing soil bacteria. The protocol can be adapted to recover bacteria from diverse niches with other desirable properties.

in-situ-isolation-culturing-recalcitrant-soil-bacteria-using-an

In Situ Isolation and Culturing of Recalcitrant Soil Bacteria using an Isolation Chip (iChip)

Ecosystems harbor millions of environmental microbes, some of them capable of producing biotechnologically relevant products such as enzymes, vitamins, ...

Institution - Indian Institute of Technology-Jodhpur

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