<p class="jove_content">We present a step-by-step protocol for high-resolution, label-free, and three-dimensional imaging of organoids using low-coherence holotomography. This protocol details organoid culture preparation, imaging acquisition, and computational image analysis, enabling real-time visualization of structural dynamics and drug responses in living organoids.</p>

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Label-free, High-Resolution 3D Imaging and Machine Learning Analysis of Intestinal Organoids via Low-Coherence Holotomography

Accurate, label-free imaging of intestinal organoids is crucial for studying their morphology, growth dynamics, and responses to environmental stimuli. ...

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