<p class="jove_content">This protocol combines flow cytometry and single-cell RNA sequencing to isolate and characterize microglial cell states in the cerebellum of early postnatal mouse brains. It uses enzymatic dissociation, Percoll centrifugation, and immunostaining to reveal microglia heterogeneity and improve understanding of their roles in cerebellar development and disease.</p>

flow-cytometry-single-cell-analysis-for-characterizing-microglia

Flow Cytometry and Single-Cell Analysis for Characterizing Microglia Activation in Early Postnatal Mouse Brain Development

Microglia, the brain's resident immune cells, exhibit region and context-specific transcriptional profiles during development and disease. This protocol ...

Institution - Azrieli Research Center of CHU Sainte-Justine

Articles Published in JoVE