<p class="jove_content">Here, we present a detailed protocol combining cryo-fixation and expansion microscopy methods (Cryo-ExM), allowing for high-resolution imaging with structural preservation adapted to various biological samples. Biological samples are frozen, embedded in a swellable polymer, denatured, expanded, and immunolabeled, enabling super-resolution imaging with standard light microscopes. </p>

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Expansion Microscopy: High-Resolution Fluorescent Imaging with a Conventional Microscope

Expansion microscopy (ExM) is an innovative super-resolution fluorescence microscopy technique that physically enlarges biological specimens, enabling ...

Institution - University of Bordeaux, CNRS, MFP, iMet UMR 5234

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