<p class="jove_content">We present a protocol to selectively permeabilize plasma membrane, enabling targeted delivery of substrates and inhibitors to assess mitochondrial oxidative phosphorylation (<strong>OXPHOS</strong>) in cultured cells, overcoming plasma membrane transport barriers that typically hinder such analysis. Using this technique, we demonstrated how OXPHOS function is modulated by muscarinic agonists and antagonists.</p>

cholinergic-liganddependent-modulation-oxidative-phosphorylation

Institution - Capillus

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