<p class="jove_content">This retrospective observational study found that a 3-week course of omega-3 PUFA-enriched ONS was associated with better short-term maintenance of WBC and NE counts, and higher body weight and BMI, compared with dietary counseling alone, suggesting potential hematological and anthropometric nutritional benefits.</p>

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Association of Omega-3 PUFA Oral Nutritional Supplementation with Hematological and Nutritional Status in Discharged Malnourished GI Cancer Patients

Short-term associations of omega-3 polyunsaturated fatty acids (PUFA)-enriched oral nutritional supplementation (ONS) with hematological, nutritional, and ...

<p class="jove_content">This protocol describes the isolation and culture of primary hepatocytes from Loxp-Stop-Loxp (LSL)-Cas9-EGFP mice and lentiviral sgRNA delivery for CRISPR/Cas9-mediated gene knockout in monolayer cultures and hepatocyte organoids. The method enables high hepatocyte yield and viability and supports efficient gene-specific knockout validation in physiologically relevant primary hepatocyte models.</p>

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Lentiviral SgRNA Delivery for CRISPR/Cas9 Editing in Cre-Dependent Cas9 Knock-in Primary Mouse Hepatocytes

Over the past few decades, CRISPR/Cas9-mediated genome editing has transformed functional studies in cell line models by making genetic manipulation ...

Department of Mechanical

Materials

Institution - University of Nottingham Ningbo China

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