<p class="jove_content">This protocol presents reflected light confocal microscopy for objective, single-cell detection of senescence-associated beta-galactosidase (SA-&#946;-gal) activity in the stromal vascular fraction isolated from human adipose tissue. The method is compatible with immunocytochemistry, incorporates pH controls for quantitative assessment, and additionally provides a more sensitive and less subjective alternative to conventional SA-&#946;-gal staining.</p>

senescence-detection-using-reflected-light-adipose-stromal-vascular

Senescence Detection Using Reflected Light in Adipose Stromal Vascular Fraction

Cellular senescence is a stress-induced state characterized by permanent cell-cycle arrest and the development of a distinctive secretory profile that ...

Institution - Nordiska Kliniken Stockholm

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