<p class="jove_content">This article introduces a multiplex bead-based flow cytometry assay for quantifying up to 12 cytokines and effector molecules from chimeric antigen receptor (CAR)-T cell-derived supernatants, enabling rapid, reproducible analysis of engineered T cell functionality with small sample volumes.</p>

bead-based-multiplex-flow-cytometry-assay-for-functional-profiling

Bead-Based Multiplex Flow Cytometry Assay for Functional Profiling of CAR T Cells

Profiling CAR T cells presents challenges due to their heterogeneity, complex immune responses, and limited sample availability. Multiplex assays enable ...

<p class="jove_content">This protocol demonstrates how dried recombinant antibody panels streamline Chimeric Antigen Receptor T (CAR T) cell characterization by reducing variability and simplifying workflows. It enables reproducible assessment of critical quality attributes, including absolute quantification of Chimeric Antigen Receptor T cells, and is compatible across different flow cytometry platforms.</p>

standardized-flow-cytometry-assay-using-dried-recombinant-antibody

Standardized Flow Cytometry Assay Using Dried Recombinant Antibody Panels for CAR T Cell Characterization

Flow cytometry is commonly used to assess critical quality attributes of Chimeric Antigen Receptor T (CAR T) cells, including viability, phenotype, and ...

Institution - IRCCS Bambino Gesù Children's Hospital

Articles Published in JoVE