<p class="jove_content">This protocol presents an optimized method for isolating the epithelial lining of the adult female mouse urethra for downstream analyses, including flow cytometry, immunostaining, and organoid culture. Methods yielded enriched, viable urethral epithelial cells, as evidenced by flow cytometry analysis, whole-mount imaging, and efficient organoid formation.</p>

an-optimized-method-to-isolate-female-mouse-urethral-epithelium-for

An Optimized Method To Isolate Female Mouse Urethral Epithelium For Single-Cell And Immunofluorescence Analysis

The urethra, lined by epithelial cells, serves as the conduit for urine outflow from the body. The epithelial lining of the urethra comprises distinct ...

Institution - BRIC-Institute for Stem Cell Science and Regenerative Medicine

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