<p class="jove_content">This protocol combines standardized seed surface sterilization with controlled germination to enable the reproducible isolation of tomato seed-associated microbiota during the seed-to-seedling transition. The approach enhances recovery of culturable microorganisms that are underrepresented in dry seeds and supports comparative culturomic studies during early seedling development.</p>

isolation-tomato-seed-associated-microbiota-through-controlled

Isolation of Tomato Seed-Associated Microbiota Through Controlled Germination and Comparative Culturing

Surface sterilization is a critical yet frequently under-optimized step in studies aiming to isolate seed-associated microorganisms, as excessively harsh ...

Institution - iPlantMicro Lab.

Articles Published in JoVE