<p class="jove_content">This protocol describes miniaturized CRISPR&#8211;Cas9 genome editing of TCR&#945;/&#946; receptor in primary human T cells using &#8216;DMF-ection&#8217;, a digital microfluidic (DMF) electroporation platform. The method enables parallel, low-input gene editing with high viability and is further adaptable for mRNA delivery into multicellular 3D spheroids.</p>

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A Digital Microfluidic Electroporation Platform for Low-Input CRISPR Genome Editing and mRNA Transfection In Suspension T Cells and 3D Cell Models

Digital microfluidic (DMF) electroporation enables precise, low-volume genetic manipulation of mammalian cells while minimizing cellular input by up to ...

Institution - DropGenie

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