<p class="jove_content">This study optimizes a Hot Phenol&#8211;based RNA extraction method for <em>Mycobacterium</em>, yielding high-quality RNA suitable for transcriptomic analyses. When benchmarked against TRIzol and RNeasy under identical conditions, the method provided higher yield, comparable integrity, and improved cost-efficiency, offering a practical alternative for large-scale gene expression studies.</p>

optimized-hot-phenolbased-rna-extraction-from-mycobacteria-robust

Optimized Hot Phenol&#8211;Based RNA Extraction from Mycobacteria: A Robust Approach for Reliable Gene Expression Analysis

Mycobacterium tuberculosis (Mtb) remains a major global health threat, underscoring the need for reliable transcriptomic studies to understand its biology ...

Institution - Amity University Uttar Pradesh

Articles Published in JoVE