<p class="jove_content">This protocol describes stepwise expression, imaging, and quantitative analysis of the vinculin TSMod FRET tension sensor to map piconewton forces in living cells. It details culture conditions, acquisition of ratiometric and spectral FRET data, image processing for accurate FRET quantification, and application to focal adhesion mechanobiology.</p>

a-step-step-protocol-for-quantifying-molecular-forces-living-cells

A Step-by-Step Protocol for Quantifying Molecular Forces in Living Cells Using the Vinculin TSMod FRET Tension Sensor

Genetically encoded FRET-based tension sensors provide a powerful approach for visualizing and quantifying molecular forces within living cells. These ...

Institution - MABLab ULR4490, Univ. Littoral Côte d’Opale

Articles Published in JoVE