This protocol describes the use of a miniaturized Montal&#8211;Mueller planar lipid bilayer system with integrated amplification to perform stable single-channel recordings. The method is demonstrated by reconstituting a purified synthetic K2P channel to validate system performance.

single-channel-electrophysiology-using-miniaturized-montalmueller

Single-Channel Electrophysiology Using a Miniaturized Montal&#8211;Mueller Planar Lipid Bilayer System for Recording a Synthetic K2P Channel

The functional reconstitution of ion channels into planar lipid bilayers provides a strategy to investigate channel activity at the single-molecule level. ...

Institution - Elements S.r.l.

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