This work presents a protocol for obtaining cloned embryos of an endangered deer species using enucleated oocytes from cattle. Here, we describe the detailed methodology of interspecific somatic cell nuclear transfer and the use of zona pellucida-free oocytes as cytoplasts. Additionally, the results obtained are discussed in detail.

interspecific-somatic-cell-nuclear-transfer-using-bovine-bos-taurus

Interspecific Somatic Cell Nuclear Transfer Using Bovine (Bos taurus) Zona Pellucida-Free Oocytes and Deer (Mazama americana) Fibroblasts

Reproductive biotechnologies used in animals make it possible to increase species populations with high genetic value. Furthermore, these technologies aid ...

Institution - State University of Ceará

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