<p class='jove_content'>In this study, we describe a straightforward method to perform Evans Blue Dye (EBD) analysis on zebrafish larvae.  This technique is a powerful tool for the characterization of skeletal muscle integrity and delineation of zebrafish models of muscular dystrophy, and is a valuable method for the development of novel therapeutics.</p>

analysis-zebrafish-larvae-skeletal-muscle-integrity-with-evans-blue

Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye

The zebrafish model is an emerging system for the study of neuromuscular disorders.  In the study of neuromuscular diseases, the integrity of the muscle ...

<p class="jove_content">Zebrafish are an emerging system for modeling human disorders of the skeletal muscle.  We describe a fast and efficient method to isolate skeletal muscle myofibers from embryonic and larval zebrafish.  This method yields a high-density myofiber preparation suitable for study of single skeletal muscle fiber morphology, protein subcellular localization, and muscle physiology.</p>

analysis-embryonic-larval-zebrafish-skeletal-myofibers-from

Analysis of Embryonic and Larval Zebrafish Skeletal Myofibers from Dissociated Preparations

The zebrafish has  proven to be a valuable model system for exploring skeletal muscle function and  for studying human muscle diseases. Despite  the many ...

Institution - Eunice Kennedy Shriver National Institute of Child Health and Human Development

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