<p class="jove_content">The biarsenical dyes FlAsH and ReAsH bind specifically to tetracysteine motifs in proteins and can selectively label proteins in live cells.  Recently this labeling strategy has been used to develop sensors for different protein conformations or oligomeric states.  We describe the labeling approach and methods to quantitatively analyze binding.</p>

reashflash-labeling-image-analysis-tetracysteine-sensor-proteins

ReAsH/FlAsH Labeling and Image Analysis of Tetracysteine Sensor Proteins in Cells

Fluorescent proteins and dyes are essential tools for the study of protein trafficking, localization and function in cells.  While fluorescent 
proteins ...

Institution - Bio21 Molecular Science and Biotechnology Institute

Articles Published in JoVE