A Cell Culture Insert-Based Protocol for Constructing a Simplified Multicellular In Vitro Blood-Tumor Barrier Model of Breast Cancer Brain Metastasis0 views•2026•Cancer ResearchBeixi Du*1, Qianran Zhang*1, Botong Li*1, Yongbo Qiu1, Xue Bai1, Mengchun Cheng2, Najin Gu3, Shida Zhu1, Xuelu Li1Beixi Du*1Qianran Zhang*1Botong Li*1Yongbo Qiu1Xue Bai1Mengchun Cheng2Najin Gu3Shida Zhu1Xuelu Li11Department of Breast Oncology and Surgery, The Second Hospital of Dalian Medical University, 2State Key Laboratory of Medical Proteomics, Dalian Institute of Chemical Physics, 3Department of Pathology, The Second Hospital of Dalian Medical UniversityDepartment of Breast Oncology and Surgery, The Second Hospital of Dalian Medical UniversityState Key Laboratory of Medical Proteomics, Dalian Institute of Chemical PhysicsDepartment of Pathology, The Second Hospital of Dalian Medical University