<p class="jove_content">Cellular viability depends on timely and efficient management of protein misfolding.  Here we describe a method for visualizing the different potential fates of a misfolded protein:  refolding, degradation, or sequestration in inclusions. We demonstrate the use of a folding sensor, Ubc9<sup>ts</sup>, for monitoring proteostasis and aggregation quality control in live cells using 4D microscopy.</p>

4d-imaging-of-protein-aggregation-in-live-cells

4D Imaging of Protein Aggregation in Live Cells

Institution - Hebrew University of Jerusalem

Articles Published in JoVE