<p class="jove_content">Here, a phosphoflow cytometry-based method is described to analyze signaling downstream of the mTORC1, JAK/STAT5, and MAPK pathways in acute human myeloid leukemia cells xenografted into mice and obtained from bone marrow aspirates. p-STAT5, p-4EBP1, p-RPS6, and p-ERK1/2 levels are simultaneously measured using a next-generation spectral flow cytometer with high sensitivity.</p>

intracellular-phosphoflow-cytometry-acute-myeloid-leukemia-patient

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

To adapt and resist approved treatments, acute myeloid leukemia (AML) cells activate specific molecular pathways that lead to changes in gene expression, ...

<p class='jove_content'>Methods to evaluate the efficacy and toxicity of RNA molecules targeting post-integration steps of the HIV-1 replication cycle are described. These methods are useful for screening new molecules and optimizing the format of existing ones. </p>

evaluation-efficacy-toxicity-rnas-targeting-hiv-1-production-for-use

Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy

Small RNA therapies targeting post-integration steps in the HIV-1 replication cycle are among the top candidates for gene therapy and have the potential ...

Institution - Lady Davis Institute for Medical Research

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