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Mycobacterial DNA Extraction using Bead Beating in Custom Buffer Followed by NGS Workflow
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2025
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Immunology and Infection1Division of Experimental Medicine, University of California, San Francisco, 2DSI-NRF Centre of Excellence for Biomedical Tuberculosis Research, SAMRC for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, 3Division of Pulmonary and Critical Care Medicine, Zuckerberg San Francisco General Hospital and Trauma Centre, University of California, San Francisco
Matrix-based DNA Extraction for Targeted Next-Generation Sequencing on Decontaminated Sputum Samples
Jennifer Williams*1, Janré Steyn*1, Emilyn Costa Conceição*1, Felicia Bernita Wells1, Melanie Grobbelaar1, Nabila Ismail1, Yonas Ghebrekristos1,2, Christoffel Johannes Opperman1,2,3,4, Sarishna Singh1,2, Jason Limberis5, Fahd Naufal5, Brendon Coenrad Mann1, Rebecca E. Colman6,7, Timothy Rodwell*6,7, Robin Mark Warren*1, John Z. Metcalfe*5
1Division of Molecular Biology and Human Genetics, SAMRC Centre for TB Research, DSI-NRF Centre of Excellence for Biomedical TB Research, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Stellenbosch University, 2National Health Laboratory Service, Green Point Tuberculosis Laboratory, 3Division of Medical Microbiology, Department of Pathology, University of Cape Town, 4Division of Medical Microbiology, Department of Pathology, University of Cape Town, 5Division of Pulmonary and Critical Care Medicine, Zuckerberg San Francisco General Hospital and Trauma Centre, University of California, San Francisco, 6Department of Medicine, University of California, San Diego, School of Medicine, 7Foundation for Innovative New Diagnostics (FIND), Campus Biotech
An Automated Microscopic Scoring Method for the γ-H2AX Foci Assay in Human Peripheral Blood Lymphocytes
1Radiation Biophysics Division, Nuclear Medicine Department, iThemba LABS, 2Department of Biotechnology, University of the Western Cape, 3Department of Medical Biosciences, University of the Western Cape, 4Division of Medical Physiology, Department of Medicine and Health Sciences, Stellenbosch University, 5Biodosimetry Service, Instituto de Investigaciones Biológicas Clemente Estable and Academic Unit on Radiation Protection, Faculty of Medicine, University of the Republic, 6MetaSystems Hard & Software GmbH
Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues
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2018
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Cited by 10
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Biochemistry1Department of Horticultural Science, Microbial and Plant Genomics Institute, University of Minnesota, 2Department of Horticultural Science, Department of Plant and Microbial Biology, Microbial and Plant Genomics Institute, University of Minnesota, 3Department of Botany and Zoology, Stellenbosch University
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