<p class="jove_content">CRISPR/Cas systems mediate adaptive immunity in Bacteria and Archaea. Many Cas proteins are proposed to act as endoribonucleases acting on crRNA precursors of varying length. Here we illustrate three different approaches to generate pre-crRNA substrates for the biochemical analysis of Cas endonuclease activity.</p>

substrate-generation-for-endonucleases-of-crisprcas-systems

Substrate Generation for Endonucleases of CRISPR/Cas Systems

The  interaction of viruses and their prokaryotic hosts shaped the evolution of  bacterial and archaeal life. Prokaryotes developed several strategies to ...

Institution - Max-Planck-Institute for terrestrial Microbiology

Articles Published in JoVE