<p class="jove_content">This protocol uses piroxicam to accelerate colitis development in the interleukin (IL-10)-knockout mouse model. The protocol ensures colitis synchronization, resulting in rigorous, reproducible results independent of sex, background strain, or vivarium conditions. It serves as a useful model for studying metabolic alterations during intestinal inflammation.</p>

a-model-piroxicam-accelerated-enterocolitis-interleukin-10-knockout

A Model of Piroxicam-Accelerated Enterocolitis in Interleukin-10 Knockout Mice for Studying Inflammation-Induced Metabolic Alterations

Inflammatory bowel disease (IBD) is a chronic inflammatory disorder of the digestive tract affecting over 10 million individuals globally. While ...

<p class='jove_content'>We describe here a novel, robust, and efficient tandem affinity purification (TAP) method for the expression, isolation, and characterization of protein complexes from eukaryotic cells. This protocol could be utilized for the biochemical characterization of discrete complexes as well as the identification of novel interactors and post-translational modifications that regulate their function.</p>

tandem-affinity-purification-protein-complexes-from-eukaryotic

Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells

The purification of active protein-protein and protein-nucleic acid complexes is crucial for the characterization of enzymatic activities and de novo ...

Institution - The University of Texas Southwestern Medical Center

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