<p class="jove_content">The Green Monster method enables the rapid assembly of multiple deletions marked with a reporter gene encoding green fluorescent protein. This method is based on driving yeast strains through repeated cycles of sexual assortment of deletions and fluorescence-based enrichment of cells carrying more deletions.</p>

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The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions

Phenotypes  for a gene deletion are often revealed only when the mutation is tested in a  particular genetic background or environmental condition1,2. ...

Institution - Mt Sinai Hospital

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