<p class="jove_content">This protocol presents an optimized two-step collagenase liver perfusion technique in a rat model and shows the use of isolated hepatocytes for <em>in vitro </em>long-term culture of 3D organoids.</p>

rat-liver-perfusion-primary-hepatocytes-isolation-an-old-procedure

Rat Liver Perfusion and Primary Hepatocytes Isolation: An Old Procedure Crucial for Cutting-Edge 3D Organoids Culture

Primary hepatocytes are a commonly used tool for in vitro liver-related studies. However, the maintenance of these cells has always been a challenge due ...

Institution - Italian Liver Foundation

Articles Published in JoVE