<p class="jove_content">This procedure demonstrates the purification and in vitro expansion of antigen specific CD4+ T cells from whole peripheral blood and their visualization using MHC class II tetramers.  Tetramers permit the direct visualization of T cells with a single antigen specificity and defined MHC class II restriction.
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visualizing-antigen-specific-cd4-t-cells-using-mhc-class-ii-tetramers

Visualizing Antigen Specific CD4+ T Cells using MHC Class II Tetramers

Major histocompatibility complex (MHC) class II tetramers allow the direct visualization of antigen specific CD4+ T cells by flow cytometry.  This method ...

Institution - Benaroya Research Institute

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