This article describes the methodology for generating SARS-CoV-2 pseudotyped particles using a luciferase reporter system and its use to identify entry inhibitors. These protocols are demonstrated using two different entry-inhibitors, a recombinant human ACE2-Fc fusion protein and the small-molecule compound arbidol.

procedures-for-identification-sars-cov-2-entry-inhibitors-as

Procedures for the Identification of SARS-CoV-2 Entry Inhibitors as Potential Antivirals using MLV-Based Pseudoviruses

The SARS-CoV-2 virus is an emergent, zoonotic pathogen that has caused a huge burden of disease and mortality around the world since its emergence in ...

The paramagnetic properties of hemozoin are used to isolate late stages of Plasmodium falciparum-infected red blood cells growing in culture. The method is simple and fast and does not affect the subsequent invasive capabilities of the parasites.

separation-plasmodium-falciparum-late-stage-infected-erythrocytes

Separation of Plasmodium falciparum Late Stage-infected Erythrocytes by Magnetic Means

Unlike other Plasmodium species, P. falciparum can be cultured in the lab, which facilitates its study 1. While the parasitemia achieved can reach the ...

Institution - Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP)

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