Here, we provide a comprehensive protocol to resolve initial local Ca2+ signals, known as Ca2+ microdomains, in primary murine and human T cells using fluorescence microscopy. This protocol serves as a valuable resource for researchers examining Ca2+ signaling pathways within immune cells and to further unravel their function.

imaging-initial-ca2-microdomains-in-primary-t-cells

Imaging Initial Ca2+ Microdomains in Primary T Cells

Local, sub-second Ca2+ signals, termed Ca2+ microdomains, are highly dynamic and short-lived Ca2+ signals, which result in a global [Ca2+]i elevation and ...

The Calcium Signalling Group

Department of Biochemistry and Molecular Cell Biology

Institution - University Medical Centre Hamburg-Eppendorf

Articles Published in JoVE