Overview

This study demonstrates the in vivo insertion of an osmotic pump for local drug delivery to ischemic hind limbs in mice. Additionally, the hind limb vasculature is perfused with Microfil for micro-computed tomography imaging.

Key Study Components

Area of Science

Neuroscience
Vascular Biology
Drug Delivery Systems

Background

Ischemia in hind limbs can be modeled in mice.
Osmotic pumps provide a method for sustained drug delivery.
Micro-computed tomography imaging aids in visualizing vascular structures.
Microfil is a silicone radiopaque agent used for perfusion.

Purpose of Study

To demonstrate the technique for osmotic pump insertion.
To establish a model of hind limb ischemia in mice.
To prepare hind limb vasculature for imaging.

Methods Used

Incision made in the shoulder and hind limb for tubing insertion.
Osmotic pump and catheter are inserted and sutured into place.
Femoral artery is double ligated to induce ischemia.
Hind limb vasculature is perfused with Microfil through the abdominal aorta.

Main Results

Successful insertion of osmotic pump for drug delivery.
Establishment of hind limb ischemia in the mouse model.
Effective perfusion of vasculature with Microfil.
Preparation for subsequent micro-CT imaging.

Conclusions

The method provides a reliable approach for local drug delivery in ischemic models.
Microfil perfusion is effective for imaging vascular structures.
This technique can facilitate further studies in vascular biology and drug delivery.

Frequently Asked Questions

What is the purpose of the osmotic pump?

The osmotic pump is used for local drug delivery to the ischemic hind limbs of mice.

How is hind limb ischemia induced in the mouse model?

Hind limb ischemia is induced by double ligating the femoral artery.

What agent is used for vascular perfusion?

Microfil, a silicone radiopaque agent, is used for vascular perfusion.

What imaging technique is utilized after perfusion?

Micro-computed tomography (micro-CT) imaging is utilized to visualize the vasculature.

What are the key steps in the procedure?

Key steps include making incisions, inserting the osmotic pump, ligating the artery, and perfusing with Microfil.

What are the implications of this study?

This study provides a method for local drug delivery and imaging in ischemic models, aiding future research.

Institution - The Texas Heart Institute at St. Luke's Episcopal Hospital

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