This protocol assesses functional synaptic activity between defined neuronal partners in vivo in the central nervous system of Drosophila melanogaster larvae using genetically encoded tools. CsChrimson-mediated optogenetic stimulation of presynaptic cIVda sensory neurons induces calcium-dependent photoconversion of CaMPARI in postsynaptic Basin-4 interneurons.

functional-characterization-individual-pre-postsynaptic-partners

Functional Characterization of Individual Pre- and Postsynaptic Partners In the Drosophila Larval Central Nervous System Using CaMPARI

Connectome studies have greatly expanded understanding of synaptic connectivity in the nervous systems of various species. While characterization of ...

Institution - University of Maryland Baltimore County (UMBC)

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