<p class="jove_content">Here we describe the stages of sample collection and preparation for RIBO-seq in bacteria. Sequencing of the libraries prepared according to these guidelines results in sufficient data for comprehensive bioinformatic analysis. The protocol we present is simple, uses standard laboratory equipment and takes seven days from lysis to obtaining libraries. </p>

ribo-seq-bacteria-sample-collection-library-preparation-protocol-for

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing

The ribosome profiling technique (RIBO-seq) is currently the most effective tool for studying the process of protein synthesis in vivo. The advantage of ...

Laboratory of Gene Expression

ECOTECH-COMPLEX

Institution - Maria Curie-Sklodowska University

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