<p class="jove_content">Three-dimensional spheroid culture systems improve upon conventional 2D models for AAV gene therapy development. This methodology enables sensitive detection of AAV transduction at low doses, enhanced physiological relevance, and robust long-term monitoring. The platform supports potency assessment, high-throughput screening, and vector optimization across diverse cell lines and AAV serotypes.</p>

evaluation-of-3d-spheroids-for-aav-transduction-studies

Evaluation of 3D Spheroids for AAV Transduction Studies

Adeno-associated viruses (AAVs) are potent vectors used for gene delivery in gene therapy products. Their development requires in vitro&#160;systems that ...

Institution - Center for Biologics Evaluation and Research

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