Using time-lapse imaging, the complete regeneration process of Hydra spheroids can be followed. This protocol describes the generation of Hydra spheroids, discusses a variety of mounting strategies, and details how to perform long-term, live imaging using spinning disk confocal microscopy as well as light-sheet fluorescence microscopy.

preparation-and-live-imaging-of-regenerating-hydra-spheroids

Preparation and Live Imaging of Regenerating Hydra Spheroids

Hydra is renowned for its exceptional regenerative capacity. Remarkably, small regenerating fragments of Hydra tissue, called Hydra spheroids, can ...

This article describes a simple and rapid protocol to evaluate the oligomeric state of the dynamin-like GTPase MxA protein from lysates of human cells using a combination of non-denaturing PAGE with western blot analysis.

characterization-multi-subunit-protein-complexes-human-mxa-using-non

Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis

The formation of oligomeric complexes is a crucial prerequisite for the proper structure and function of many proteins. The interferon-induced antiviral ...

Here we describe the experimental procedures involved in two-photon imaging of mouse cortex during behavior in a virtual reality environment.

two-photon-calcium-imaging-mice-navigating-virtual-reality

Two-photon Calcium Imaging in Mice Navigating a Virtual Reality Environment

In recent years, two-photon imaging has become an invaluable tool in neuroscience, as it allows for chronic measurement of the activity of genetically ...

Institution - Friedrich Miescher Institute for Biomedical Research

Articles Published in JoVE