<p class="jove_content">Experimental artifacts, including fluctuations in cell viability and assay interference, hinder accurate quantification of glycerol release in lipid-rich primary adipocytes. To mitigate these challenges, an MTT-based normalization strategy is used to adjust glycerol measurements for cell metabolic activity, thereby enhancing the reliability of lipolysis quantification.</p>

mtt-based-normalization-glycerol-release-for-accurate-quantification

Institution - Putian University

Articles Published in JoVE