<p class="jove_content">In this article, we present two complementary methods to measure specific rates of translational error and mistranslation in the model mycobacterium, <em>Mycobacterium smegmatis</em>, using gain-of-function reporter systems. The methods can be employed to measure accurate error rates in low throughput or relative error rates in a more high-throughput setting.</p>

measurement-specific-mycobacterial-mistranslation-rates-with-gain

Measurement of Specific Mycobacterial Mistranslation Rates with Gain-of-function Reporter Systems

The translation of genes into proteins is prone to errors. Although the average rate of translational error in model systems is estimated to be 1/10,000 ...

Institution - Tsinghua University School of Medicine

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