<p class="jove_content">Endogenous retrovirus-derived cis-regulatory elements regulate early embryonic gene expression. This protocol describes their CRISPR-Cas9-mediated deletion in human naive pluripotent stem cells to assess their roles in gene regulation and peri-implantation developmental processes.</p>

genetic-deletion-cis-regulatory-elements-to-dissect-function-non

Genetic Deletion of Cis-Regulatory Elements to Dissect the Function of the Non-coding Genome in human Preimplantation Models

Cis-regulatory elements coordinate gene expression in a spatially and temporally controlled manner and contribute to the establishment of distinct ...

<p class="jove_content">The quality of mouse embryonic fibroblasts (MEFs) is dictated by the right strain of mouse such as CF-1. Pluripotency-supportive MEFs and conditioned media (CM) obtained from these should contain optimal concentrations of Activin A, Gremlin and Tgf&beta;1 needed for the Activin/Nodal and FGF pathways to co-operatively maintain self-renewal and pluripotency.</p>

preparation-mouse-embryonic-fibroblast-cells-suitable-for-culturing

Preparation of Mouse Embryonic Fibroblast Cells Suitable for Culturing Human Embryonic and Induced Pluripotent Stem Cells

In general, human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs)1 can be cultured under variable conditions. However, it ...

Institution - Max Planck Institute for Molecular Genetics

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