We describe a protocol for generating cornea organoids from iPSCs derived from aniridia patients, creating a patient-specific model to study corneal disorders. These organoids mimic key structural and functional features, providing a reliable in vitro platform for investigating disease mechanisms, testing potential treatments, and advancing personalized regenerative approaches for anterior eye diseases.

patient-specific-ipsc-derived-cornea-organoids-for-investigating

Patient-Specific iPSC-Derived Cornea Organoids for Investigating Aniridia-Associated Corneal Disorders

Aniridia is a rare congenital eye disorder marked by the partial or complete absence of the iris, and it is often associated with corneal opacification ...

This protocol describes the generation of fast and reproducible endodermal hepatic organoids (eHEPOs). With this protocol, eHEPOs can be produced within 2 weeks and expand long-term (more than 1 year) without losing their differentiation and functionality.

generation-of-functional-endodermal-hepatic-organoids

Generation of Functional Endodermal Hepatic Organoids

Organoid technology has allowed us to generate a variety of human organ-like mini structures, such as for the liver, brain, and intestine, in vitro. The ...

Institution - Izmir Biomedicine and Genome Center

Articles Published in JoVE