<p class="jove_content">This protocol describes deoxyribonucleic acid extraction, metabarcoding library preparation, Oxford Nanopore long-read sequencing, and bioinformatic analysis of bat guano and urine samples to characterize microbial communities and detect potential pathogens, enabling taxonomic profiling of prokaryotic, archaeal, and eukaryotic microorganisms.</p>

oxford-nanopore-long-read-sequencing-bat-guano-urine-for-microbiome

Oxford Nanopore Long-Read Sequencing of Bat Guano and Urine for Microbiome Characterization and Pathogen Detection

Deoxyribonucleic acid (DNA) sequencing has made possible the study of genetic sequences for taxonomic assignment, mutational analyses, and novel organism ...

<p class="jove_content">Here, we present a simple technique to assess environmental antimicrobial resistance (AMR) by enhancing the proportion of low-molecular-weight extracellular DNA. Prior treatment with 20%-30% PEG and 1.2 M NaCl allows detection of both genomic and horizontally transferred AMR genes. The protocol lends itself to a kit-free process with additional optimization.</p>

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Enhanced Extraction of Low-Molecular Weight DNA from Wastewater for Comprehensive Assessment of Antimicrobial Resistance

Environmental surveillance is recognized as an important tool for assessing public health in the post-pandemic era. Water, in particular wastewater, has ...

Institution - Ashoka University

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