<p class="jove_content">The present protocol describes the seeding and staining of neuronal mitochondria in microfluidic chambers. The fluidic pressure gradient in these chambers allows for the selective treatment of mitochondria in axons to analyze their properties in response to pharmacological challenges without affecting the cell body compartment.</p>

microfluidics-assisted-selective-depolarization-of-axonal-mitochondria

Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria

Mitochondria are the primary suppliers of ATP (adenosine triphosphate) in neurons. Mitochondrial dysfunction is a common phenotype in many ...

<p class="jove-content">Here we describe the experimental procedures involved in two-photon imaging of mouse cortex during behavior in a virtual reality environment.</p>

two-photon-calcium-imaging-mice-navigating-virtual-reality

Two-photon Calcium Imaging in Mice Navigating a Virtual Reality Environment

In recent years, two-photon imaging has become an invaluable tool in neuroscience, as it allows for chronic measurement of the activity of genetically ...

Institution - Max Planck Institute of Neurobiology

Articles Published in JoVE