<p class="jove_content">Here we demonstrate a simple protocol to create a random mutant library for a given target sequence. We show how this method, which is performed in vivo in Escherichia coli, can be coupled with functional selections to evolve new enzymatic activities. </p>

mutagenesis-functional-selection-protocols-for-directed-evolution

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in <em>E. coli</em>

The efficient generation of genetic diversity represents an invaluable molecular tool that can be used to label DNA synthesis, to create unique molecular ...

bacterial-gene-expression-analysis-using-microarrays

Bacterial Gene Expression Analysis Using Microarrays

vibrio-cholerae-model-organism-to-study-bacterial-pathogenesis

Institution - University of California Santa Cruz - UCSC

Articles Published in JoVE