This protocol describes a colorimetric method to reliably assess the influence of chemicals on Deiodinase 1 (DIO1) activity in human liver microsomes using the Sandell-Kolthoff reaction. It includes predefined acceptance criteria and statistical performance metrics and supports ongoing efforts toward OECD test guideline inclusion.

colorimetric-assessment-deiodinase-1-activity-human-liver-microsomes

Colorimetric Assessment of Deiodinase 1 Activity in Human Liver Microsomes Using the Sandell-Kolthoff Reaction

This method enables the identification of test substances that inhibit DIO1 activity and may thus interfere with thyroid hormone homeostasis. The protocol ...

This protocol describes in particular the extraction of total IgY from egg yolk by means of polyethylene glycol precipitation and gives general information about IgY technology.

igy-technology-extraction-chicken-antibodies-from-egg-yolk

IgY Technology: Extraction of Chicken Antibodies from Egg Yolk by Polyethylene Glycol (PEG) Precipitation

Hens can be immunized by means of  i.m. vaccination (Musculus pectoralis, left and right, injection volume  0.5-1.0 ml) or by means of Gene-Gun ...

Measuring the yaw torque of tethered Drosophila with the torque meter allows the neuroscientist exquisite control of the stimulus situation of the experimental animal. Together with the unique genetic tools available in the fruit fly, this paradigm is used for a wide variety of neurobiological research.

operant-learning-of-drosophila-at-the-torque-meter

Operant Learning of Drosophila at the Torque Meter

For experiments at the torque meter, flies are kept on standard fly medium at 25&deg;C and 60% humidity with a 12hr light/12hr dark regime. A standardized ...

Institution - Free University of Berlin

Articles Published in JoVE