<p class="jove_content">This protocol describes a method for mapping pre-mRNA 3&#39; end processing sites.</p>

3-end-sequencing-library-preparation-with-a-seq2

3' End Sequencing Library Preparation with A-seq2

Studies in the last decade have revealed a complex and dynamic variety of pre-mRNA cleavage and polyadenylation reactions. mRNAs with long 3&#39; ...

<p class="jove_content">RNA transcripts are subject to extensive posttranscriptional regulation that is mediated by a multitude of trans-acting RNA-binding proteins (RBPs). Here we present a generalizable method to identify precisely and on a transcriptome-wide scale the RNA binding sites of RBPs.</p>

par-clip-method-to-identify-transcriptome-wide-binding-sites-rna

PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins

RNA transcripts are subjected to post-transcriptional gene regulation by interacting with hundreds of RNA-binding proteins (RBPs) and microRNA-containing ...

Institution - Biozentrum der Universität Basel and Swiss Institute of Bioinformatics (SIB)

Articles Published in JoVE